human kyse150 cells  (DSMZ)

Journal: iScience

Article Title: KRT15 identified by scRNA-Seq and machine learning as stemness regulator and prognostic biomarker in ESCC

doi: 10.1016/j.isci.2026.115020

Figure Lengend Snippet: KRT15 is enriched in ESCC tumor spheroids and positively correlates with the stemness marker CD44 (A) Stem cell enrichment experiments performed in four cell lines, with images captured over time. (B and C) qPCR analysis of CD44 and KRT15 mRNA expression in tumorspheres versus adherent cells from ECA109, KYSE180, KYSE150, and TE1 cell lines. n = 3, statistical analysis performed using an unpaired t test. Data are represented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (D) Western blot analysis of CD44 and KRT15 protein expression in tumor spheres compared to adherent cells in ECA109 and KYSE180 cell lines.

Article Snippet: Human: KYSE150 cells , DSMZ , ACC-375.

Techniques: Marker, Expressing, Western Blot

Journal: Scientific Reports

Article Title: Overexpression of LINC01354 predicts well prognosis and suppresses esophageal cancer progression

doi: 10.1038/s41598-025-20455-2

Figure Lengend Snippet: High tumor expression of LINC01354 holds diagnostic potential in ESCA. ( A ) A comparative analysis of LINC01354 expression and prognosis revealed that LINC01354 was decreased in ESCA and BRCA and led to poor prognosis in patients. ( B , C ) The high expression of LINC01354 in ESCA was validated in additional two cohorts. ( D ) Paired analysis showed that LINC01354 was down-regulated in 95% of ESCA patients in GSE53622 cohort. ( E ) Further analysis depicted that LINC01354 was decreased in 89.9% of ESCA patients in GSE53624 cohort. ( F , G ) ROC analysis was used to evaluate the diagnostic potential of LINC01354 in the GSE53622 (95% confidence interval: 0.8317 to 0.9544) and GSE53624 (95% confidence interval: 0.8374 to 0.9277) cohorts. ( H ) The expression of LINC01354 in Het-1 A, TE1, KYSE70, KYSE150, and KYSE450 cells was examined by qRT-PCR, ** p < 0.01, *** p < 0.001, independent-samples T test. ( I ) FISH was employed to detect the expression of LINC01354 in ESCA TMA, scale bar = 800 μm.

Article Snippet: Human ESCA cell lines KYSE150 and TE1 were purchased from Procell (Wuhan, China).

Techniques: Expressing, Diagnostic Assay, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Overexpression of LINC01354 predicts well prognosis and suppresses esophageal cancer progression

doi: 10.1038/s41598-025-20455-2

Figure Lengend Snippet: LINC01354 is involved in cell-cycle regulation and curbs tumorigenesis in ESCA. ( A , B ) Functional enrichment analysis of LINC01354 associated genes in ESCA revealed the biological function of LINC01354. ( C – E ) The effect of overexpression of LINC01354 on ESCA tumor growth was evaluated in a nude mouse model, ** p < 0.01. ( F , G ) The inhibition of LINC01354 on KYSE150 cell cycle process was validated by flow cytometry analysis, **** p < 0.0001.

Article Snippet: Human ESCA cell lines KYSE150 and TE1 were purchased from Procell (Wuhan, China).

Techniques: Functional Assay, Over Expression, Inhibition, Flow Cytometry

Journal: Drug Design, Development and Therapy

Article Title: Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

doi: 10.2147/DDDT.S470061

Figure Lengend Snippet: Alkannin inhibits the proliferation of ESCC cells. ( A ) Chemical structure of Alkannin. ( B ) Cell viability of KYSE150 and Eca109 cells treated with different concentrations of Alkannin for 24, 48, and 72 h. (C) Colony formation assay of KYSE150 and Eca109 cells after treatment with Alkannin for 24 h. (D) Western blot detects the PCNA protein levels of KYSE150 and Eca109 cells after treatment with Alkannin for 24 h. Data are expressed as means ± SD from five independent experiments, *P < 0.05; ***P < 0.001 compared to control by one-way ANOVA.

Article Snippet: The human ESCC cell lines KYSE150 and Eca109 (Procell Biosciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, United States) supplemented with 10% fetal bovine serum (BI, Israel), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, United States).

Techniques: Colony Assay, Western Blot, Control

Journal: Drug Design, Development and Therapy

Article Title: Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

doi: 10.2147/DDDT.S470061

Figure Lengend Snippet: Alkannin inhibits the migration and invasion of ESCC cells. (A) Wound healing assay shows the mobility of KYSE150 and Eca109 cells after treatment with Alkannin. (B) Transwell assay detects the invasion ability of KYSE150 and Eca109 cells after treatment with Alkannin. (C) Western blot analyzes the protein expression of MMP2, MMP9, Vimentin, and β-Catenin in KYSE150 and Eca109 cells after treatment with Alkannin. Data are expressed as means ± SD from five independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001 compared to control by one-way ANOVA.

Article Snippet: The human ESCC cell lines KYSE150 and Eca109 (Procell Biosciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, United States) supplemented with 10% fetal bovine serum (BI, Israel), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, United States).

Techniques: Migration, Wound Healing Assay, Transwell Assay, Western Blot, Expressing, Control

Journal: Drug Design, Development and Therapy

Article Title: Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

doi: 10.2147/DDDT.S470061

Figure Lengend Snippet: Alkannin induces apoptosis in ESCC cells. (A) Hoechst 33342 staining shows the extent of nuclear morphology of KYSE150 and Eca109 cells after treatment with different concentrations of Alkannin for 24 h. (B) Annexin V-FITC/PI assay is used to evaluate the apoptotic ratio of KYSE150 and Eca109 cells after treatment with Alkannin for 24 h. (C) Western blot analyzes the protein levels of BAX and Bcl-2 in KYSE150 and Eca109 cells. Data are expressed as means ± SD from five independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001 compared to control by one-way ANOVA.

Article Snippet: The human ESCC cell lines KYSE150 and Eca109 (Procell Biosciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, United States) supplemented with 10% fetal bovine serum (BI, Israel), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, United States).

Techniques: Staining, Western Blot, Control

Journal: Drug Design, Development and Therapy

Article Title: Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

doi: 10.2147/DDDT.S470061

Figure Lengend Snippet: Alkannin induces G2/M phase arrest in ESCC cells. (A) Flow cytometry analysis shows cell cycle distribution of KYSE150 and Eca109 cells after treatment with Alkannin for 24 h. (B) Western blot analyzes the protein levels of CyclinB1 and CDK1 in KYSE150 and Eca109 cells after treatment with Alkannin for 24 h. Data are expressed as means ± SD from five independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001 compared to control by one-way ANOVA.

Article Snippet: The human ESCC cell lines KYSE150 and Eca109 (Procell Biosciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, United States) supplemented with 10% fetal bovine serum (BI, Israel), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, United States).

Techniques: Flow Cytometry, Western Blot, Control

Journal: Drug Design, Development and Therapy

Article Title: Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

doi: 10.2147/DDDT.S470061

Figure Lengend Snippet: Transcriptomic analysis predicts the signal pathway of Alkannin anti-ESCC. (A) Volcano plot of differential gene expression (n = 4). Blue: downregulated, red: upregulated, gray: non-significant. (B) Heat map of differentially expressed genes. (C) Gene Set Enrichment Analysis (GSEA) map of the G2/M phase gene sets. (D) Gene Ontology (GO) enrichment analysis of pathways in the top 20. The red boxes indicate the most significantly enriched pathways for the G2/M phase and cell cycle that appear frequently. (E) KEGG enrichment analysis of the top 20 signaling pathways, with the red boxes highlighting the highest-ranked pathways and the cell cycle pathway ranked first.

Article Snippet: The human ESCC cell lines KYSE150 and Eca109 (Procell Biosciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, United States) supplemented with 10% fetal bovine serum (BI, Israel), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, United States).

Techniques: Gene Expression, Protein-Protein interactions

Journal: Drug Design, Development and Therapy

Article Title: Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

doi: 10.2147/DDDT.S470061

Figure Lengend Snippet: Alkannin has anti-ESCC effect via targeting GSK3β. (A) Molecular docking of Alkannin with GSK3β. (B) Immunohistochemistry analyzes the protein levels of p-GSK3β in xenograft tumor tissues. (C) Western blot analyzes the protein levels of GSK3β, p-GSK3β, and p21 in KYSE150 and Eca109 cells treated with Alkannin. Data are expressed as means ± SD from five independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001 compared to control by one-way ANOVA.

Article Snippet: The human ESCC cell lines KYSE150 and Eca109 (Procell Biosciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, United States) supplemented with 10% fetal bovine serum (BI, Israel), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, United States).

Techniques: Immunohistochemistry, Western Blot, Control

Journal: Drug Design, Development and Therapy

Article Title: Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

doi: 10.2147/DDDT.S470061

Figure Lengend Snippet: Alkannin inhibits the growth of ESCC xenograft tumors. (A) The flowchart of the ESCC xenograft tumor establishment and Alkannin intervention. (B) The changes of the tumor volume after treatment with Alkannin. (C) Representative images of tumors in different groups (n = 6). (D) The weight of xenograft tumors in different groups. (E) HE staining and immunohistochemistry analyzes the Ki67 and p-GSK3β protein expression. Data are expressed as means ± SD from six independent experiments, ***P < 0.001 compared to control by one-way ANOVA.

Article Snippet: The human ESCC cell lines KYSE150 and Eca109 (Procell Biosciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, United States) supplemented with 10% fetal bovine serum (BI, Israel), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, United States).

Techniques: Staining, Immunohistochemistry, Expressing, Control